Lab Protocols
Protocols used by the CompBio group:
Labeled Probe Hybridization
Aminoallyl Labeling of RNA for Microarrays
Amplification of Orientation-Specific RNA
RNA Extraction from Human/Mouse Cell Lines
RNA Extraction from Human or Animal Tissue
Real Time RT-PCR
Poly A+ RNA Isolation from Total RNA via Dynabeads Oligo (dT)25
Template Miniprepping
PCR Purification and Storage
Printing in DMSO
Microarray Slide Staining Procedure
Creating a Microarray Study Binder
High Throughput AminoAllyl Labelling
Growing and Purifying shRNA Low Copy Plasmids for Transfection
Lentivirus Concentration
Lentivirus Infection of Target Cells
Lentivirus Production and Transfection Protocol
RNA Isolation and Purification from Cell Lines Using Qiagen
Total and miRNA Extraction from Human Tissue Samples using TRIzol
Total RNA Extraction from Human Tissue Samples using TRIzol