Lab Protocols

Protocols used by the CompBio group.

• Labeled Probe Hybridization (pdf)
  
• Aminoallyl Labeling of RNA for Microarrays (pdf)
  
• Amplification of Orientation-Specific RNA (pdf)
  
• RNA Extraction from Human/Mouse Cell Lines (pdf)
  
• RNA Extraction from Human or Animal Tissue (pdf)
  
• Real Time RT-PCR (pdf)
  
• Poly A+ RNA Isolation from Total RNA via Dynabeads® Oligo (dT)25
  
• Template Miniprepping
  
• PCR Purification and Storage
  
• Printing in DMSO
  
• Microarray Slide Staining Procedure
  
• Creating a Microarray Study Binder
  
• High Throughput AminoAllyl Labelling
  
• Growing and Purifying shRNA Low Copy Plasmids for Transfection
  
• Lentivirus Concentration
  
• Lentivirus Infection of Target Cells
  
• Lentivirus Production and Transfection Protocol
  
• RNA Isolation and Purification from Cell Lines Using Qiagen
  
• Total and miRNA Extraction from Human Tissue Samples using TRIzol
  
• Total RNA Extraction from Human Tissue Samples using TRIzol